FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nuf, DCTN1-p150
FlyBase ID
FBig0000107792
Interaction Network
Interactions Browser links
nuf network
DCTN1-p150 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0233695-11.PD.WB
Reference
Calero-Cuenca et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nuf 

Nuf

bait

GST tag

DCTN1-p150 

Dynactin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mutation of nuf\'s aPKC phosphosite (Ser155) did not affect binding to DCTN1-p150. Also, aPKC did not compete with DCTN1-p150 for binding to nuf.

(Calero-Cuenca et al., 2016)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Calero-Cuenca et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)