FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Lk6, eIF4E1
FlyBase ID
FBig0000107677
Interaction Network
Interactions Browser links
Lk6 network
eIF4E1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0183823-1.coIP.WB
Reference
Arquier et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Lk6 

Lk6

bait

FLAG tag

eIF4E1 

eIF4E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Arquier et al., 2005)
FBrf0183823-2.coIP.WB
Reference
Arquier et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

MYC tag

eIF4E1 
Lk6 

Lk6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E1 
MAPK binding sequence
mutation decreasing interaction
aa 251

S251A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Arquier et al., 2005)
FBrf0187148-3.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lk6 

LK6

enzyme

MYC tag

Lk6 

LK6

enzyme

MYC tag

eIF4E1 

eIF4E

enzyme target

P[32] label

eIF4E1 

eIF4E

enzyme target

P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0187148-4.coIP.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lk6 

LK6

enzyme

MYC tag

Lk6 

LK6

enzyme

MYC tag

eIF4E1 

eIF4E

enzyme target

P[32] label

eIF4E1 

eIF4E

enzyme target

P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)