Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.
Proteins immunopurified with an antibody specific to the 32B isoform.
Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.
Proteins immunopurified with an antibody specific to the 32A isoform.
Interaction in vitro; proteins produced by in vitro translation.
Heterocomplexes of BEAF-32 32A and 32B isoforms have different DNA-binding properties than homocomplexes.
Various combinations of BEAF-32 32A and 32B isoforms were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.
Interaction in vitro; proteins produced and labeled by in vitro translation.
Proteins immunopurified with an antibody specific to the 32A isoform.
Interaction in vitro; proteins produced and labeled by in vitro translation.
Proteins immunopurified with an antibody specific to the 32B isoform.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was embryonic nuclear extracts of transgenic fly line; bait produced from endogenous gene; prey produced from transgenic construct.
weak dimerization, "BESS" construct, coordinates relative to BEAF-32-PB
strong dimerization, "LZB" construct, coordinates relative to BEAF-32-PB
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
"BESS" construct, coordinates relative to BEAF-32-PB
Source was cell extract of bacterial cells; bait produced from recombinant construct; prey produced from recombinant construct.
Source was cell extract of S2 cell line; protein produced from transfected construct.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was larval eye discs of transgenic fly line; proteins produced from tagged transgenic construct.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD. Positive control.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library made from equal quantities of polyA RNA from embryo, larvae, adult).
Source was live S2 cells; proteins produced from transfected constructs.
Positive control.
