FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Myb, E2f2
FlyBase ID
FBig0000107573
Interaction Network
Interactions Browser links
Myb network
E2f2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0180259-24.coIP.WB
Reference
Lewis et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myb 

Myb

bait
E2f2 

E2F2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lewis et al., 2004)

All immunoprecipitations were performed in the presence of ethidium bromide in order to disrupt protein DNA interactions.

(Lewis et al., 2004)
FBrf0200827-12.coIP.WB
Reference
Beall et al., 2007
Description
physical association
Assay
Collection
Source/Stage
testis / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E2f2 

E2F2

bait
Myb 

Myb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was testis of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Beall et al., 2007)
FBrf0202994-5.coIP.WB
Reference
Wen et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Myb 

Myb

bait

FLAG tag

Myb 
E2f2 

E2F2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myb 
C-terminal domain
sufficient binding region
unspecified

C-terminal fragment of Myb lacking the DNA binding domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wen et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)