Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Arr2 can be pelleted with ninaE in blue light and released efficiently to the supernatant in a blue followed by orange light regimen. The release of Arr2 into the supernatant is defective in CDase[1] mutants.

Source was adult heads of wild-type, mutant or transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

A greater percentage of Arr2 binds to ninaE in a norpA mutant background than in wild-type. The concomitant induction of autophagy in a norpA mutant background reduces the percentage of Arr2 bound to ninaE to near wild-type levels.

Source was adult head membranes of wild-type fly line; bait produced from endogenous gene; prey produced and labeled by in vitro translation.

Arr2 doesn\'t bind to ninaE mutant membranes.

Binding dependent upon light exposure.

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.