Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Precipitations were done in the presence of ethidium bromide to rule out DNA mediated protein protein interaction.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

All immunoprecipitations were performed in the presence of ethidium bromide in order to disrupt protein DNA interactions.