FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mys, rhea
FlyBase ID
FBig0000106161
Interaction Network
Interactions Browser links
mys network
rhea network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0193518-1.PBD.FM
Reference
Tanentzapf et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mys 

diβ

bait

chimeric with torso

mys 
mys 
mys 
mys 
mys 
rhea 

talinH

prey

GFP tag

rhea 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mys 
cytoplasmic domain
sufficient binding region
AILLLWKLLTTIHDRREFARFEKERMNAKWDTGENPIYKQATSTFKNPMYAGK
mys 
membrane proximal region residues 807-810
mutation disrupting interaction
aa 807-810

D807V, E810E, coordinates relative to mys-PA

mys 
membrane proximal region residues 811-817
mutation disrupting interaction
aa 811-817

F811A, F814L, E817V, coordinates relative to mys-PA

mys 
NPXY motif residue N828
mutation disrupting interaction
aa 828

N828A, coordinates relative to mys-PA

mys 
NPXY motif residue P829
mutation disrupting interaction
aa 829

P829A, coordinates relative to mys-PA

rhea 
talin head domain
sufficient binding region
aa 1-454

coordinates relative to rhea-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The chimeric form of mys combined the mys cytoplasmic tail with the extracellular and transmembrane domains of constitutively active tor to confer oligomerization. The ability of this mys chimera to recruit tagged rhea to muscle attachment sites was taken as evidence of direct physical interaction.

(Tanentzapf et al., 2006)

Source was fixed embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Tanentzapf et al., 2006)
FBrf0255068-2.coIP.WB
Reference
Orr et al., 2022
Description
physical association
Assay
Collection
Source/Stage
brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mys 

Mys

bait

YFP tag

mys 
rhea 

Talin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mys 
interaction domain residue 796
mutation disrupting interaction
aa 796

coordinates relative to mys-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brains of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Orr et al., 2022)
FBrf0255068-5.coIP.WB
Reference
Orr et al., 2022
Description
physical association
Assay
Collection
Source/Stage
brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mys 

Mys

bait

YFP tag

mys 
rhea 

Talin

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mys 
interaction domain residue 211
mutation increasing interaction
aa 211

coordinates relative to mys-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brains of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenously tagged transgenic construct.

(Orr et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)