FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nanos, tral
FlyBase ID
FBig0000105733
Interaction Network
Interactions Browser links
nanos network
tral network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0212721-6.PD.WB
Reference
Jeske et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 2embryonic stage 5embryonic stage 3embryonic stage 4embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

nos

bait

biotin label

nanos 
tral 

Trailer Hitch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3 prime UTR
sufficient binding region
nt 7-161
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced by in vitro translation.

(Jeske et al., 2011)
FBrf0236765-12.PD.MS
Reference
Götze et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

nos

bait

biotin label

nanos 
nanos 
tral 

Tral

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3-prime UTR
sufficient binding region
nt 7-161
nanos 
SRE
mutation decreasing interaction
CTGGC

both SREs in nos 3-prime UTR changed from CTGGC to CTCGC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type embryo extract.

(Götze et al., 2017)

Binding dependent on RNA length with stoichiometry of approximately one per 100nt.

(Götze et al., 2017)
FBrf0236765-5.PD.WB
Reference
Götze et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

nos

bait

biotin label

nanos 
nanos 
tral 

Tral

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3-prime UTR
sufficient binding region
nt 7-161
nanos 
SRE
mutation decreasing interaction
CTGGC

both SREs in nos 3-prime UTR changed from CTGGC to CTCGC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type embryo extract.

(Götze et al., 2017)

Binding dependent on RNA length with stoichiometry of approximately one per 100nt.

(Götze et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)