FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ana2, Sas-6
FlyBase ID
FBig0000105631
Interaction Network
Interactions Browser links
ana2 network
Sas-6 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0209887-1.coIP.WB
Reference
Stevens et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sas-6 

DSas-6

bait
ana2 

Ana2

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Stevens et al., 2010)
FBrf0209887-3.Y2H
Reference
Stevens et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

bait

fused to GAL4 DNA-binding domain

ana2 
Sas-6 

DSas-6

prey

fused to GAL4 activation domain

Sas-6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana2 
C-terminal region
sufficient binding region
aa 201-420

coordinates relative to ana2-PA

Sas-6 
N-terminal region
sufficient binding region
aa 1-210

coordinates relative to Sas-6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Stevens et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Stevens et al., 2010)
FBrf0226888-7.PD.A
Reference
Dzhindzhev et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

bait

GST tag

ana2 
ana2 
Sas-6 

Sas6

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana2 
C-terminal region
sufficient binding region
aa 281-420

"Ana2-C" construct, coordinates relative to ana2-PA

ana2 
Plk4 phosphorylation sites
mutation disrupting interaction
aa 318-373

construct "4A", S318A, S365A, S370A and S373A, coordinates relative to ana2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ana2 was treated with functional or kinase-dead SAK to assess the effects of SAK phosphorylation on ana2 binding of other proteins.

(Dzhindzhev et al., 2014)

All four ana2 phosphorylated residues seem to contribute to Sas-6 binding since mutation of individual sites diminished but did not abolish the interaction.

(Dzhindzhev et al., 2014)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Dzhindzhev et al., 2014)
FBrf0226888-8.coIP.WB
Reference
Dzhindzhev et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

bait

FLAG tag

ana2 
Sas-6 

Sas6

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana2 
Plk4 phosphorylation sites
mutation disrupting interaction
aa 318-373

construct "4A", S318A, S365A, S370A and S373A, coordinates relative to ana2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dzhindzhev et al., 2014)
FBrf0233282-138.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

bait

fused to GAL4 DNA-binding domain

ana2 
Sas-6 

Sas-6

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana2 
C-terminal region
sufficient binding region
aa 177-420

coordinates relative to ana2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)

This interaction is detected only when ana2 is mutated to mimic the SAK phosphorylated state (construct 3DE).

(Galletta et al., 2016)
FBrf0237625-2.PD.A
Reference
Dzhindzhev et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

bait

GST tag

Sas-6 

Sas6

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription/translation.

(Dzhindzhev et al., 2017)
FBrf0250142-1.PD.WB
Reference
Fatalska et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

bait

GST tag

ana2 
Sas-6 

Sas6

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana2 
Phosphomimetic STAN motif serines 318; 365; 370 and 373
mutation increasing interaction
aa S318D, S365D, S370D and S373D

coordinates relative to ana2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Fatalska et al., 2020)
FBrf0250142-2.CH.MW
Reference
Fatalska et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

neutral component
ana2 

Ana2

neutral component
ana2 

Ana2

neutral component
Sas-6 

Sas6

neutral component
Sas-6 

Sas6

neutral component
Sas-6 

Sas6

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Fatalska et al., 2020)
FBrf0250142-3.PD.WB
Reference
Fatalska et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

bait

GST tag

ana2 
Sas-6 

Sas6

prey

MBP tag

Sas-6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana2 
interaction domain residues 325-331
mutation decreasing interaction
aa 325-331

coordinates relative to ana2-PA

Sas-6 
interaction domain residues 385-410
necessary binding region
aa 385-398

coordinates relative to Sas-6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Fatalska et al., 2020)
FBrf0250142-4.coIP.WB
Reference
Fatalska et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

bait

FLAG tag

ana2 
Sas-6 

Sas6

prey

MYC tag

Sas-6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana2 
Phosphomimetic STAN motif serines 318; 365; 370 and 373
mutation increasing interaction
aa S318D, S365D, S370D and S373D

coordinates relative to ana2-PA

Sas-6 
interaction domain residues 385-410
necessary binding region
aa 385-410

coordinates relative to Sas-6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fatalska et al., 2020)
FBrf0255743-7.PD.A
Reference
Ábrahám et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

bait

GST tag

ana2 
Sas-6 

Sas6

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana2 
STAN motif
sufficient binding region
aa 281-420
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Constitutively active or non-degradable SAK co-expressed with active or inactive PpD3 was able to phosphorylate ana2 which is required for interaction with Sas-6.

(Ábrahám et al., 2023)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Ábrahám et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)