FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sas-4, polo
FlyBase ID
FBig0000105626
Interaction Network
Interactions Browser links
Sas-4 network
polo network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0233282-127.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

fused to GAL4 DNA-binding domain

Sas-4 
polo 

Polo

prey

fused to GAL4 activation domain

polo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sas-4 
N-terminal region
sufficient binding region
aa 1-347

coordinates relative to Sas-4-PA

polo 
C-terminal region
sufficient binding region
aa 340-576

coordinates relative to polo-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)