FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Lk6
FlyBase ID
FBig0000105610
Interaction Network
Interactions Browser links
Lk6 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0187148-1.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lk6 

LK6

enzyme

MYC tag

Lk6 

LK6

enzyme target

P[32] label

Lk6 

LK6

enzyme

MYC tag

Lk6 

LK6

enzyme target

P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0233282-108.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lk6 

Lk6

bait

fused to GAL4 DNA-binding domain

Lk6 

Lk6

prey

fused to GAL4 activation domain

Lk6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lk6 
N-terminal region
sufficient binding region
aa 1-498

coordinates relative to Lk6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 0 )
References (2)