Sas-4
fused to GAL4 DNA-binding domain
fused to GAL4 activation domain
coordinates relative to Sas-4-PA
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
The N-terminal and central regions of Sas-4 homodimerize. The C-terminal region is also able to interact with the full length Sas-4 construct.