FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cep135
FlyBase ID
FBig0000105499
Interaction Network
Interactions Browser links
Cep135 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0233282-133.coIP.WB
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cep135 

Cep135

bait

GFP tag

Cep135 

Cep135

prey

FLAG tag

Cep135 
Cep135 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cep135 
N-terminal region
sufficient binding region
aa 1-490

coordinates relative to Cep135-PA

Cep135 
C-terminal region
sufficient binding region
aa 491-1059

coordinates relative to Cep135-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Galletta et al., 2016)

The N- and C-terminal regions each show homodimerization interactions.

(Galletta et al., 2016)
FBrf0233282-78.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cep135 

CEP135/Bld10

bait

fused to GAL4 DNA-binding domain

Cep135 

CEP135/Bld10

prey

fused to GAL4 activation domain

Cep135 
Cep135 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cep135 
C-terminal region
sufficient binding region
aa 491-1059

coordinates relative to Cep135-PA

Cep135 
N-terminal region
sufficient binding region
aa 1-490

coordinates relative to Cep135-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)

The N- and C-terminal regions each show homodimerization interactions.

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 0 )
References (1)