FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Plp, ana2
FlyBase ID
FBig0000105490
Interaction Network
Interactions Browser links
Plp network
ana2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0233282-40.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Plp 

PLP

bait

fused to GAL4 DNA-binding domain

Plp 
ana2 

Ana2

prey

fused to GAL4 activation domain

ana2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana2 
C-terminal region
sufficient binding region
aa 177-420

coordinates relative to ana2-PA

Plp 
region F3
sufficient binding region
aa 1377-1811

coordinates relative to Plp-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)

Weak interaction.

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)