SAK, ana2 Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

SAK, ana2

FlyBase ID

FBig0000105430

Interaction Network

Interactions Browser links

SAK network
ana2 network

esyN Network Diagram

Reported Interactions

FBrf0226888-1.ENZ.MS

Reference

Description

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SAK

Plk4

enzyme

MBP tag

SAK

Plk4

enzyme

MBP tag

ana2

Ana2

enzyme target

GST tag, P[32] label

ana2

Ana2

enzyme target

GST tag, P[32] label

ana2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

FBrf0226888-2.ENZ.A

Reference

Description

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SAK

Plk4

enzyme

MBP tag

SAK

Plk4

enzyme

MBP tag

ana2

Ana2

enzyme target

GST tag, P[32] label

ana2

Ana2

enzyme target

GST tag, P[32] label

ana2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

FBrf0233282-42.Y2H

Reference

Galletta et al., 2016

Description

physical association

Assay

two hybrid

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SAK

Plk4/SAK

bait

fused to GAL4 DNA-binding domain

SAK

ana2

Ana2

prey

fused to GAL4 activation domain

ana2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

ana2

C-terminal region

sufficient binding region

aa 177-420

coordinates relative to ana2-PA

SAK

N-terminal region

sufficient binding region

aa 1-381

coordinates relative to SAK-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)

FBrf0237625-1.ENZ.A

Reference

Description

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SAK

Plk4

enzyme

MBP tag

SAK

Plk4

enzyme

MBP tag

SAK

ana2

Ana2

enzyme target

[35]S label

ana2

Ana2

enzyme target

[35]S label

ana2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

FBrf0250617-1.coIP.WB

Reference

McLamarrah et al., 2018

Description

physical association

Assay

anti tag coimmunoprecipitation

anti tag western blot

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

SAK

Plk4

bait

GFP tag

SAK

ana2

Ana2

prey

V5 tag

ana2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

ana2

CC domain

necessary binding region

aa 191-230

coordinates relative to ana2-PA

ana2

phosphomimetic residues

necessary binding region

aa 63,69,150,159,242

amino acids mutated to aspartate or glutamate; coordinates relative to ana2-PA

SAK

PB3 domain

sufficient binding region

aa 659-741

coordinates relative to SAK-PA

SAK

interaction domain

sufficient binding region

aa 1-317

coordinates relative to SAK-PA

SAK

interaction domain

mutation disrupting interaction

aa 232-317

in addition to PB3 deletion, lowers interaction significantly; coordinates relative to SAK-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(McLamarrah et al., 2018)

ana2 oligomerizes and was thus depleted by RNAi to eliminate its effect on the interaction with SAK; kinase dead forms of SAK interact more strongly with ana2.

(McLamarrah et al., 2018)

FBrf0250617-2.Y2H

Reference

McLamarrah et al., 2018

Description

physical association

Assay

two hybrid

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SAK

Plk4

bait

fused to GAL4 DNA-binding domain

SAK

ana2

Ana2

prey

fused to VP16 activation domain

ana2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

ana2

CT domain

necessary binding region

aa 177-420

coordinates relative to ana2-PA

SAK

PB3 domain

sufficient binding region

aa 603-769

coordinates relative to SAK-PA

SAK

interaction domain

sufficient binding region

aa 1-381

coordinates relative to SAK-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(McLamarrah et al., 2018)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(McLamarrah et al., 2018)

FBrf0250617-3.ENZ.MS

Reference

Description

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SAK

Plk4

enzyme

FLAG-His tag

SAK

Plk4

enzyme

FLAG-His tag

ana2

Ana2

enzyme target

GST tag

ana2

Ana2

enzyme target

GST tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

FBrf0250617-3.PD.WB

Reference

McLamarrah et al., 2018

Description

physical association

Assay

pull down

anti tag western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

ana2

Ana2

bait

GST tag

SAK

Plk4

prey

FLAG tag

SAK

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

SAK

interaction domain

sufficient binding region

aa 1-317

coordinates relative to SAK-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

(McLamarrah et al., 2018)

FBrf0255743-6.ENZ.MW

Reference

Description

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SAK

Plk4

enzyme

MBP tag

SAK

Plk4

enzyme

MBP tag

ana2

Ana2

enzyme target

GST tag

ana2

Ana2

enzyme target

GST tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

External Crossreferences and Linkouts ( 1 )

Linkouts

MIST - An integrated Molecular Interaction Database

SAK,ana2

References (5)

Report Sections

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