FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ana1, Cp110
FlyBase ID
FBig0000105423
Interaction Network
Interactions Browser links
ana1 network
Cp110 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0233282-12.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana1 

Ana1

bait

fused to GAL4 DNA-binding domain

ana1 
Cp110 

CP110 Fragment 1

prey

fused to GAL4 activation domain

Cp110 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cp110 
N-terminal region
sufficient binding region
aa 1-325

coordinates relative to Cp110-PA

ana1 
C-terminal region
sufficient binding region
aa 1311-1729

coordinates relative to ana1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
FBrf0233282-13.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp110 

CP110 Fragment 1

bait

fused to GAL4 DNA-binding domain

Cp110 
ana1 

Ana1

prey

fused to GAL4 activation domain

ana1 
ana1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cp110 
N-terminal region
sufficient binding region
aa 1-325

coordinates relative to Cp110-PA

ana1 
central region
sufficient binding region
aa 876-1310

coordinates relative to ana1-PA

ana1 
C-terminal region
sufficient binding region
aa 1311-1729

coordinates relative to ana1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)