FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cp110, Cep97
FlyBase ID
FBig0000105412
Interaction Network
Interactions Browser links
Cp110 network
Cep97 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0233282-86.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cep97 

CEP97

bait

fused to GAL4 DNA-binding domain

Cep97 
Cp110 

CP110 Fragment 1

prey

fused to GAL4 activation domain

Cp110 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cep97 
C-terminal region
sufficient binding region
aa 514-806

coordinates relative to Cep97-PB

Cp110 
N-terminal region
sufficient binding region
aa 1-325

coordinates relative to Cp110-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
FBrf0233282-87.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp110 

CP110 Fragment 1

bait

fused to GAL4 DNA-binding domain

Cp110 
Cep97 

CEP97

prey

fused to GAL4 activation domain

Cep97 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cep97 
C-terminal region
sufficient binding region
aa 514-806

coordinates relative to Cep97-PB

Cp110 
N-terminal region
sufficient binding region
aa 1-325

coordinates relative to Cp110-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
FBrf0257757-5.coIP.WB
Reference
Ryniawec et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cep97 

Cep97

bait

GFP tag

Cep97 
Cp110 

Cp110

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cep97 
interaction domain
sufficient binding region
aa 514-806

coordinates relative to Cep97-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ryniawec et al., 2023)
FBrf0257757-6.coIP.WB
Reference
Ryniawec et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cp110 

Cp110

bait

GFP tag

Cp110 
Cep97 

Cep97

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cp110 
N terminal domain
sufficient binding region
aa 1-325

coordinates relative to Cp110-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ryniawec et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)