FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
vap, Dscam1
FlyBase ID
FBig0000104877
Interaction Network
Interactions Browser links
vap network
Dscam1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0225482-7.coIP.MS
Reference
Rowshanravan et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
vap 

RasGAP

bait

GFP tag, His tag

vap 
vap 
Dscam1 

Dscam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vap 
SH2 domain 1
mutation decreasing interaction
aa 110

R110L

vap 
SH2 domain 2
mutation decreasing interaction
aa 278

R278L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Rowshanravan et al., 2014)

Mutation of both vap SH2 domains abolishes the interaction with Dscam1.

(Rowshanravan et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)