FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
spri, vap
FlyBase ID
FBig0000104866
Interaction Network
Interactions Browser links
spri network
vap network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0225482-1.coIP.MS
Reference
Rowshanravan et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
vap 

RasGAP

bait

GFP tag, His tag

vap 
vap 
spri 

sprint

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vap 
SH2 domain 1
mutation decreasing interaction
aa 110

R110L

vap 
SH2 domain 2
mutation decreasing interaction
aa 278

R278L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Rowshanravan et al., 2014)

Mutation of both vap SH2 domains abolishes the interaction with spri.

(Rowshanravan et al., 2014)
FBrf0225482-2.coIP.WB
Reference
Rowshanravan et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
spri 

sprint

bait

GFP tag, His tag

spri 
spri 
vap 

RasGAP

prey

Myc tag

vap 
vap 
vap 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vap 
SH2 and SH3 domains
sufficient binding region
aa 83-343
spri 
central region
sufficient binding region
aa 443-1292

coordinates relative to spri-PE

spri 
central region
necessary binding region
aa 812-1292

coordinates relative to spri-PE

vap 
SH2 domain 1
mutation decreasing interaction
aa 110

R110L

vap 
SH2 domain 2
mutation decreasing interaction
aa 278

R278L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rowshanravan et al., 2014)

Mutation of both vap SH2 domains abolishes the interaction with spri - the vap SH3 domain is dispensable. Observed spri phosphotyrosine residues (Y1056, Y363) also appear to be dispensable for the interaction.

(Rowshanravan et al., 2014)
FBrf0225482-3.PD.WB
Reference
Rowshanravan et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
vap 

RasGAP

bait

GST tag

vap 
vap 
vap 
spri 

sprint

prey

GFP tag, His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vap 
SH2 and SH3 domains
sufficient binding region
aa 83-343
vap 
SH2 domain 1
mutation decreasing interaction
aa 110

R110L

vap 
SH2 domain 2
mutation decreasing interaction
aa 278

R278L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Rowshanravan et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)