FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
tud, Pyk
FlyBase ID
FBig0000104520
Interaction Network
Interactions Browser links
tud network
Pyk network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0227705-1.PD.WB
Reference
Gao et al., 2015
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pyk 

Pyk

bait

GFP tag

tud 

Tudor

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of female transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Gao et al., 2015)

Binding of aub peptide to tud protein inhibits interaction between tud and PyK

(Gao et al., 2015)
FBrf0227705-2.PD.WB
Reference
Gao et al., 2015
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pyk 

Pyk

bait

GFP tag

tud 

Tudor

prey

HA tag

tud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
mini tud delta three
sufficient binding region

A 1271 amino acid smaller form of tud with a 1244 amino acid deletion from Leu301 to Arg1544.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of female transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Gao et al., 2015)

Binding of aub peptide to tud protein inhibits interaction between tud and PyK

(Gao et al., 2015)
FBrf0234826-5.coIP.MS
Reference
Zheng et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 2
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

Tud

bait

HA tag

Pyk 

Pyruvate kinase

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zheng et al., 2016)

Embryos were formaldehyde crosslinked before affinity purification.

(Zheng et al., 2016)
FBrf0259220-1.PD.WB
Reference
Wahiduzzaman et al., 2024
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pyk 

Pyk

bait

GFP tag

tud 

Tud

prey

HA tag

tud 
tud 
tud 
tud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
tudor domain 1-2
sufficient binding region
aa 1-585
tud 
tudor domain 7-8
sufficient binding region
aa 1605-1964
tud 
tudor domain 8-9
sufficient binding region
aa 1791-2160
tud 
tudor domain 10-11
sufficient binding region
aa 2140-2515
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and purified from tagged transgenic construct in ovary extract.; prey produced as a recombinant fusion protein in bacterial system.

(Wahiduzzaman et al., 2024)
FBrf0259220-3.PD.WB
Reference
Wahiduzzaman et al., 2024
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pyk 

Pyk

bait

GFP tag

tud 

Tud

prey

His-Sumo tag

tud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
tudor domain 7 and 10
sufficient binding region
aa 1605-1785 and aa 2140-2340
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and purified from tagged transgenic construct in ovary extract.

(Wahiduzzaman et al., 2024)

Pyk does not interact with single tud domain 7 or 10, but when they are mixed together, the interaction is strong.

(Wahiduzzaman et al., 2024)
FBrf0259220-4.PD.WB
Reference
Wahiduzzaman et al., 2024
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

Tud

bait

HA tag

tud 
Pyk 

Pyk

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
tudor domain 7-11
sufficient binding region
aa 1605-2515
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and purified from tagged transgenic construct in ovary extract.

(Wahiduzzaman et al., 2024)

aub competes with Pyk for binding to tud domains 7-11.

(Wahiduzzaman et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)