FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000104475
Interaction Network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0229620-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Obsc 

NTAP-Kin1 or 2

bait

SBP tag

Obsc 
Obsc 
mask 

MASK1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Obsc 
kinase domain 1
sufficient binding region
Obsc 
kinase domain 2
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from transgenic gene.

FBrf0229620-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Obsc 

Kin1 or 2

neutral component
Obsc 

Kin1 or 2

neutral component
Obsc 
Obsc 
Obsc 

Kin1 or 2

neutral component
Obsc 
Obsc 
mask 

MASK1

neutral component
mask 

MASK1

neutral component
mask 

MASK1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Obsc 
kinase domain 1
sufficient binding region
Obsc 
kinase domain 2
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)