FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
asl, Cep97
FlyBase ID
FBig0000104456
Interaction Network
Interactions Browser links
asl network
Cep97 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0232405-1.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
asl 

Asl

bait

fused to GAL4 DNA-binding domain

asl 
Cep97 

Cep97

prey

fused to GAL4 activation domain

Cep97 
Cep97 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
asl 
C-terminal region
sufficient binding region
aa 626-993
Cep97 
C-terminal region
sufficient binding region
aa 514-806

coordinates relative to Cep97-PB

Cep97 
N-terminal region
sufficient binding region
aa 1-351

coordinates relative to Cep97-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)
FBrf0232405-2.coIP.WB
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
asl 

Asl

bait

GFP tag

asl 
Cep97 

Cep97

prey

FLAG tag

Cep97 
Cep97 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
asl 
C-terminal region
sufficient binding region
aa 626-993
Cep97 
C-terminal region
sufficient binding region
aa 514-806

coordinates relative to Cep97-PB

Cep97 
N-terminal region
sufficient binding region
aa 1-351

coordinates relative to Cep97-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Galletta et al., 2016)
FBrf0232405-3.coIP.WB
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cep97 

Cep97

bait

GFP tag

asl 

Asl

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Galletta et al., 2016)
FBrf0233282-55.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cep97 

CEP97

bait

fused to GAL4 DNA-binding domain

Cep97 
Cep97 
asl 

Asl

prey

fused to GAL4 activation domain

asl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
asl 
C-terminal region
sufficient binding region
aa 626-994

coordinates relative to asl-PA

Cep97 
N-terminal region
sufficient binding region
aa 1-351

coordinates relative to Cep97-PB

Cep97 
C-terminal region
sufficient binding region
aa 514-806

coordinates relative to Cep97-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)

Weak interaction.

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)