FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pc, Su(z)2
FlyBase ID
FBig0000104260
Interaction Network
Interactions Browser links
Pc network
Su(z)2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0206952-14.coIP.WB
Reference
Lo et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pc 

Pc

bait
Su(z)2 

Su(Z)2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lo et al., 2009)
FBrf0206952-2.CH.WB
Reference
Lo et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(z)2 

Su(Z)2

bait

FLAG tag

Su(z)2 
Su(z)2 
Pc 

Pc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(z)2 
N-terminal domain
sufficient binding region
aa 1-217
Su(z)2 
N-terminal domain
necessary binding region
aa 1-217
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins derived from nuclear extract of recombinant baculovirus infected Sf9 cells.

(Lo et al., 2009)
FBrf0206952-20.coIP.WB
Reference
Lo et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Su(z)2 

Su(Z)2

bait
Pc 

Pc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lo et al., 2009)
FBrf0206952-26.coIP.WB
Reference
Lo et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Pc 

Pc

bait
Su(z)2 

Su(Z)2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lo et al., 2009)
FBrf0206952-32.coIP.WB
Reference
Lo et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc-unspecified
Participants
Corresponds to
Reported as
Role
Note
Su(z)2 

Su(Z)2

bait
Pc 

Pc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lo et al., 2009)
FBrf0206952-38.coIP.WB
Reference
Lo et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc-unspecified
Participants
Corresponds to
Reported as
Role
Note
Pc 

Pc

bait
Su(z)2 

Su(Z)2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lo et al., 2009)
FBrf0206952-8.coIP.WB
Reference
Lo et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(z)2 

Su(Z)2

bait
Pc 

Pc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lo et al., 2009)
FBrf0213360-9.PD.MS
Reference
Strübbe et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pc 

Pc

bait

BioTag

Su(z)2 

Su(z)2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The BirA biotin ligase was coexpressed to biotinylate Pc bait in vivo, which was affinity purified using streptavidin beads and eluted by cleaving the TEV site between the bait and BioTag

(Strübbe et al., 2011)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Strübbe et al., 2011)
FBrf0228678-6.TAP.MS
Reference
Kang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pc 

Pc

bait

BioTAP tag

Su(z)2 

Su(z)2

prey

89 peptide counts

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Kang et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)