FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Gas41, Dcr-1
FlyBase ID
FBig0000104178
Interaction Network
Interactions Browser links
Gas41 network
Dcr-1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0227240-1.coIP.WB
Reference
Gandhi et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dcr-1 

DICER1

bait
Dcr-1 
Gas41 

GAS41

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dcr-1 
interaction domain
mutation disrupting interaction
aa 1147

Q1147X

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult cell extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Gandhi et al., 2015)
FBrf0227240-2.coIP.WB
Reference
Gandhi et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gas41 

GAS41

bait
Dcr-1 

DICER1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult cell extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Gandhi et al., 2015)
FBrf0227240-3.PD.WB
Reference
Gandhi et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dcr-1 

DICER1

bait

GST

Dcr-1 
Dcr-1 
Dcr-1 
Dcr-1 
Dcr-1 
Gas41 

Gas41

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dcr-1 
interaction domain 1
sufficient binding region
aa 516 - 606

Helicase C domain

Dcr-1 
interaction domain 2
sufficient binding region
aa 825 - 920

dsRNA binding domain

Dcr-1 
interaction domain 3
sufficient binding region
aa 1096 - 1269

PAZ domain

Dcr-1 
interaction domain 4
sufficient binding region
aa 1742 - 1919

RNase 1 domain

Dcr-1 
interaction domain 5
sufficient binding region
aa 2029 - 2150

RNase 2 domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from endogenous gene.

(Gandhi et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)