Source was embryo of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type or transgenic fly line; proteins derived from tagged transgenic construct or endogeonous genes.
Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct or endogenous gene.
Interaction in vitro; protein produced in a baculovirus and insect cell system.
Crystal structure of hexameric complex captured as 15 pairwise interactions.
Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Embryonic nuclear extract was treated with DNase and RNase.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Embryonic nuclear extract was treated with DNase and RNase.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control.
Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
The ORC complex visualized by EM was captured as 15 pairwise interactions. The addition of ATP caused the complex to adopt an active/open conformation.
Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and High5 cell system.
Comparison of EM structures of ORC complex, ORC complex bound to DNA and a ternary complex consisting of ORC, DNA and Cdc6 curated as a series on binary protein-protein interactions.
Interaction in vitro; proteins produced as recombinant fusion proteins in a baculovirus and insect cell system.
Source was ovaries of transgenic, Orc6 mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.
