FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sas-4, Cep135
FlyBase ID
FBig0000103865
Interaction Network
Interactions Browser links
Sas-4 network
Cep135 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0223945-4.coIP.WB
Reference
Zheng et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

dSas-4

bait

Td-tomato tag

Sas-4 
Sas-4 
Cep135 

Bld-10

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sas-4 
TCP domain β9-10 surface-residue 792
mutation decreasing interaction
aa 792

E792V, "TCP-1" construct

Sas-4 
TCP domain β9-10 surface-residues 800-802
mutation decreasing interaction
aa 800-802

V800A, I802A, "TCP-2" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of C131 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Zheng et al., 2014)

This interaction is not affected by the L841K mutation.

(Zheng et al., 2014)

Constructs were expressed in acentrosomal cell line derived from Sas-4 null homozygous mutant flies.

(Zheng et al., 2014)
FBrf0223945-7.PD.WB
Reference
Zheng et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

GST tag

Sas-4 
Sas-4 
Sas-4 
Cep135 

Bld-10

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sas-4 
TCP domain
sufficient binding region
aa 710-897
Sas-4 
TCP domain β9-10 surface-residue 792
mutation decreasing interaction
aa 792

E792V, "TCP-1" construct

Sas-4 
TCP domain β9-10 surface-residues 800-802
mutation decreasing interaction
aa 800-802

V800A, I802A, "TCP-2" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Zheng et al., 2014)

The Sas-4 TCP domain interacts with Cep135 and ana2, but not with γ-tubulin.

(Zheng et al., 2014)
FBrf0223945-8.PD.WB
Reference
Zheng et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

GST tag

Sas-4 
Sas-4 
Sas-4 
Cep135 

Ana-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sas-4 
TCP domain
sufficient binding region
aa 710-897
Sas-4 
TCP domain β9-10 surface-residue 792
mutation decreasing interaction
aa 792

E792V, "TCP-1" construct

Sas-4 
TCP domain β9-10 surface-residues 800-802
mutation decreasing interaction
aa 800-802

V800A, I802A, "TCP-2" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Zheng et al., 2014)

The Sas-4 TCP domain interacts with Cep135 and ana2, but not with γ-tubulin.

(Zheng et al., 2014)
FBrf0233282-141.coIP.WB
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cep135 

Cep135

bait

GFP tag

Cep135 
Sas-4 

Sas4

prey

FLAG tag

Sas-4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cep135 
N-terminal region
sufficient binding region
aa 1-490

construct "F1", coordinates relative to Cep135-PA

Sas-4 
N-terminal region
sufficient binding region
aa 1-347

construct "F1", coordinates relative to Sas-4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Galletta et al., 2016)
FBrf0233282-84.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

fused to GAL4 DNA-binding domain

Sas-4 
Cep135 

CEP135/Bld10

prey

fused to GAL4 activation domain

Cep135 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sas-4 
N-terminal region
sufficient binding region
aa 1-347

coordinates relative to Sas-4-PA

Cep135 
N-terminal region
sufficient binding region
aa 1-490

coordinates relative to Cep135-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)