Interaction in vitro; proteins derived from transfected S2 cell extract.

When co-transfected, ana1, asl and Cep135 perfectly co-sediment in the same fractions. Co-sedimentation of asl and Cep135 is not observed in teh absence of ana1, suggesting that it bridges asl and Cep135.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The C-terminal region of Cep135 binds the central region of asl. Also, the N-terminal region of Cep135 binds the C-terminal region of asl, but only when Cep135 carries mutations that mimic the SAK (Plk4) phosphorylated state (nine Thr and Ser residues mutated to Asp or Glu, construct "9DE").

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).