FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ana1, Cep135
FlyBase ID
FBig0000103829
Interaction Network
Interactions Browser links
ana1 network
Cep135 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0230510-1.PD.A
Reference
Fu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana1 

Ana1

bait

MBP tag

Cep135 

Cep135

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Fu et al., 2016)
FBrf0230510-3.coIP.WB
Reference
Fu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ana1 

Ana1

bait

GFP tag

ana1 
Cep135 

Cep135

prey

FLAG tag

Cep135 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana1 
N-terminal region
sufficient binding region
aa 1-935
Cep135 
N-terminal region
sufficient binding region
aa 1-510

coordinates relative to Cep135-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fu et al., 2016)
FBrf0230510-6.CS.MW
Reference
Fu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ana1 

Ana1

neutral component

FLAG tag

ana1 

Ana1

neutral component

FLAG tag

ana1 

Ana1

neutral component

FLAG tag

Cep135 

Cep135

neutral component

GFP tag

Cep135 

Cep135

neutral component

GFP tag

Cep135 

Cep135

neutral component

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins derived from transfected S2 cell extract.

(Fu et al., 2016)

When co-transfected, ana1, asl and Cep135 perfectly co-sediment in the same fractions. Co-sedimentation of asl and Cep135 is not observed in teh absence of ana1, suggesting that it bridges asl and Cep135.

(Fu et al., 2016)
FBrf0233282-8.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana1 

Ana1

bait

fused to GAL4 DNA-binding domain

ana1 
Cep135 

CEP135/Bld10

prey

fused to GAL4 activation domain

Cep135 
Cep135 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana1 
C-terminal region
sufficient binding region
aa 1311-1729

coordinates relative to ana1-PA

Cep135 
N-terminal region
sufficient binding region
aa 1-490

coordinates relative to Cep135-PA

Cep135 
C-terminal region
sufficient binding region
aa 491-1059

coordinates relative to Cep135-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
FBrf0233282-9.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cep135 

CEP135/Bld10

bait

fused to GAL4 DNA-binding domain

Cep135 
ana1 

Ana1

prey

fused to GAL4 activation domain

ana1 
ana1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cep135 
N-terminal region
sufficient binding region
aa 1-490

coordinates relative to Cep135-PA

ana1 
central region
sufficient binding region
aa 876-1310

coordinates relative to ana1-PA

ana1 
C-terminal region
sufficient binding region
aa 1311-1729

coordinates relative to ana1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)