FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hook, Rab11
FlyBase ID
FBig0000103813
Interaction Network
Interactions Browser links
hook network
Rab11 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0224129-1.coIP.WB
Reference
Szatmári et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hook 

Hook

bait

HA tag

Rab11 

Rab11

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Szatmári et al., 2014)

The strength of the hook-Rab11 interaction was increased upon starvation, presumably due to the induction of autophagy.

(Szatmári et al., 2014)
FBrf0224129-2.coIP.WB
Reference
Szatmári et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab11 

Rab11

bait

HA tag

hook 

Hook

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of D.mel-2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Szatmári et al., 2014)
FBrf0224129-3.coIP.WB
Reference
Szatmári et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hook 

Hook

bait

FLAG tag

Rab11 

Rab11

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Szatmári et al., 2014)

hook binds wild-type Rab11, but not a GDP-locked variant of Rab11.

(Szatmári et al., 2014)
FBrf0224129-4.coIP.WB
Reference
Szatmári et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab11 

Rab11

bait

FLAG tag

hook 

Hook

prey

HA tag

hook 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hook 
coiled coil domain
sufficient binding region
aa 180-530

"CC" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of D.mel-2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Szatmári et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)