FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eve, gro
FlyBase ID
FBig0000103796
Interaction Network
Interactions Browser links
eve network
gro network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0135733-2.PD.A
Reference
Kobayashi et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eve 

Eve

bait

His tag

eve 
eve 
eve 
eve 
gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eve 
C-terminal region
sufficient binding region
aa 238-376
eve 
LFKPY motif
necessary binding region
KLFKPYKTEA

"Δ" construct

eve 
LFKPY motif residue 371
mutation disrupting interaction
aa 371

P371A

eve 
LFKPY motif residue 372
mutation disrupting interaction
aa 372

Y371A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kobayashi et al., 2001)
FBrf0135733-4.Y2H
Reference
Kobayashi et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eve 

eve

bait

fused to GAL4 DNA-binding domain

eve 
eve 
eve 
eve 
gro 

gro

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eve 
C-terminal region
sufficient binding region
aa 238-376
eve 
LFKPY motif
necessary binding region
KLFKPYKTEA

"Δ" construct

eve 
LFKPY motif residue 371
mutation disrupting interaction
aa 371

P371A

eve 
LFKPY motif residue 372
mutation disrupting interaction
aa 372

Y371A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kobayashi et al., 2001)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Kobayashi et al., 2001)
FBrf0211008-4.Y2H
Reference
Braid et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eve 

Eve

bait

fused to GAL4 DNA-binding domain

gro 

Gro

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Braid et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Braid et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)