FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Crag, Cam
FlyBase ID
FBig0000103761
Interaction Network
Interactions Browser links
Crag network
Cam network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0105342-14.FW.TV
Reference
Xu et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Crag 

CRAG

bait

GST tag

Crag 
Cam 

Calmodulin

prey

biotin label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Crag 
calmodulin-binding site
sufficient binding region
YSLYFLILPSMVLDPRHAGKEPEILRAAYDVLVRASRLKITCDEFCYRIMMQLCGIHNLPVLAVRLHYLMKRSGVQANALTYGFYNRCVLESQWPQDSTTISQIRWNRIKNVVLGAAQFRKAGKQRAASKVNKSLSASQDQNLSTLETVDGQSRTSLASSSGDGGGHGLL
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey obtained from commercial source.

(Xu et al., 1998)
FBrf0105342-17.PD.WB
Reference
Xu et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

Calmodulin

bait

coupled to agarose beads

Crag 

CRAG

prey

GST tag

Crag 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Crag 
calmodulin-binding site
sufficient binding region
YSLYFLILPSMVLDPRHAGKEPEILRAAYDVLVRASRLKITCDEFCYRIMMQLCGIHNLPVLAVRLHYLMKRSGVQANALTYGFYNRCVLESQWPQDSTTISQIRWNRIKNVVLGAAQFRKAGKQRAASKVNKSLSASQDQNLSTLETVDGQSRTSLASSSGDGGGHGLL
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait obtained from commercial source; prey produced as a recombinant fusion protein in bacterial system.

(Xu et al., 1998)
FBrf0105342-7.LPD
Reference
Xu et al., 1998
Description
physical association
Assay
Collection
Source/Stage
retina / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

Calmodulin

bait

[125]I label

Crag 

CRAG

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

3 Crag clones recovered in this phage display screen for Cam binding proteins.

(Xu et al., 1998)

Interaction in vitro; bait obtained from commercial source; prey produced as transgenic fusion protein in bacteriophage (prey from adult retina cDNA expression library).

(Xu et al., 1998)
FBrf0204713-1.coIP.WB
Reference
Denef et al., 2008
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Crag 

Crag

bait

HA tag

Cam 

CaM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Denef et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)