FB2024_04 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Abi, SCAR
FlyBase ID
FBig0000103754
Interaction Network
Interactions Browser links
Abi network
SCAR network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0188146-1.Y2H
Reference
Bogdan et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

unspecified role
Abi 

Abi

unspecified role
Abi 
Abi 

Abi

unspecified role
Abi 
SCAR 

WAVE

unspecified role
SCAR 

WAVE

unspecified role
SCAR 

WAVE

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abi 
N-terminal region
necessary binding region
aa 1-85
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bogdan et al., 2005)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Bogdan et al., 2005)
FBrf0188146-10.coIP.WB
Reference
Bogdan et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

bait
SCAR 

WAVE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bogdan et al., 2005)
FBrf0209286-9.coIP.WB
Reference
Fricke et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

bait
SCAR 

WAVE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Fricke et al., 2009)
FBrf0212496-1.FC.FM
Reference
Gohl et al., 2010
Description
physical association
Assay
Collection
Source/Stage
photoreceptor / third instar larval stagewing disc / third instar larval stagewing / pupal stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
SCAR 

WAVE

neutral component

YFP tag, N-terminal fragment

SCAR 

WAVE

neutral component

YFP tag, N-terminal fragment

SCAR 

WAVE

neutral component

YFP tag, N-terminal fragment

Abi 

Abi

neutral component

YFP tag, C-terminal fragment

Abi 

Abi

neutral component

YFP tag, C-terminal fragment

Abi 

Abi

neutral component

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval cells; proteins produced from transfected constructs.

(Gohl et al., 2010)

SCAR-Abi interaction is enriched at the basal side of the pupal wing.

(Gohl et al., 2010)
FBrf0216499-1.CH.WB
Reference
Stephan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SCAR 

WAVE

neutral component
SCAR 

WAVE

neutral component
SCAR 

WAVE

neutral component
Abi 

Abi

neutral component
Abi 

Abi

neutral component
Abi 

Abi

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins derived from adult head extract.

(Stephan et al., 2011)

A large fraction of Abi cofractionates with SCAR and the WAVE complex subunit Hem in protein complexes of about 400-500 kDa.

(Stephan et al., 2011)
FBrf0226180-5.coIP.WB
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

bait

GFP tag

SCAR 

WAVE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Chen et al., 2014)
FBrf0242076-1.PD.WB
Reference
Kim et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

bait

GST tag

Abi 
Abi 
SCAR 

SCAR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abi 
interaction domain
sufficient binding region
aa 1-387

coordinates relative to Abi-PA

Abi 
WAB domain
necessary binding region
aa 30-65

coordinates relative to Abi-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2R+ cell extract.

(Kim et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)