FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
TBPH, futsch
FlyBase ID
FBig0000103555
Interaction Network
Interactions Browser links
TBPH network
futsch network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0213270-1.coIP.PCR
Reference
Godena et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
TBPH 

TDP-43

bait

FLAG tag

TBPH 
futsch 

Futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
TBPH 
RNA binding domain
mutation disrupting interaction
aa 150-152

F150L, F152L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Godena et al., 2011)
FBrf0226844-1.coIP.PCR
Reference
Coyne et al., 2014
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
TBPH 

TDP-43

bait

YFP tag

futsch 

futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction of TBPH with futsch RNA is not affected by the TBPH G298S mutation.

(Coyne et al., 2014)

Source was adult heads; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Coyne et al., 2014)
FBrf0233248-1.EMSA.A
Reference
Romano et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
TBPH 

TBPH

bait

GST tag

futsch 

futsch

prey

[32]P label

futsch 
futsch 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
futsch 
5-prime UTR
sufficient binding region
UAACAAGGGAACACCCAGAGUAUACCCUCUUUUUUUUCGAGUGUGCGAACCAGUUUUUUUUGUGUGUGACCCAAACCAAAGGCAAGAACAACAGCUACAACUGCACCAACUGUGCCAUGGAAG
futsch 
5-prime UTR UG-rich sequence
necessary binding region
GUGUGCGAACCAGUUUUUUUUGUGUGUG

"Δ"UG construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Romano et al., 2016)
FBrf0233248-2.PD.PCR
Reference
Romano et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
TBPH 

TBPH

bait

GST tag

futsch 

futsch

prey
futsch 
futsch 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
futsch 
5-prime UTR
sufficient binding region
UAACAAGGGAACACCCAGAGUAUACCCUCUUUUUUUUCGAGUGUGCGAACCAGUUUUUUUUGUGUGUGACCCAAACCAAAGGCAAGAACAACAGCUACAACUGCACCAACUGUGCCAUGGAAG
futsch 
5-prime UTR UG-rich sequence
necessary binding region
GUGUGCGAACCAGUUUUUUUUGUGUGUG

"Δ"UG construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro transcription.

(Romano et al., 2016)

TBPH increases the expression of futsch 5\'UTR luciferase reporters. This requires the UG-rich sequence.

(Romano et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)