Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).
Two-hybrid system: yeast LexA-BD/B42-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
ΔV616, coordinates relative to vas-PA
ΔP617, coordinates relative to vas-PA
ΔD618, coordinates relative to vas-PA
Two-hybrid system: yeast LexA-BD/B42-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
