FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
yki, Ncoa6
FlyBase ID
FBig0000102957
Interaction Network
Interactions Browser links
yki network
Ncoa6 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0226375-1.coIP.WB
Reference
Qing et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Ncoa6 

Ncoa6

bait

FLAG tag

Ncoa6 
yki 

yki

prey

HA tag

yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ncoa6 
PPxY motifs
mutation disrupting interaction
aa 263-1465

Y263A, Y663A, Y1465A ("3m")

yki 
WW domains
mutation disrupting interaction
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Qing et al., 2014)

Coexpression of Ncoa6 and yki induces nuclear accumulation of yki.

(Qing et al., 2014)
FBrf0233980-1.coIP.WB
Reference
Oh et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

V5 tag

yki 
Ncoa6 

Ncoa6

prey

FLAG tag

Ncoa6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW domain
mutation disrupting interaction
aa 292,295,361,364

W292A,P295A,W361A,P364A, coordinates relative to yki-PH

Ncoa6 
interaction domain
mutation disrupting interaction
aa 262,632,1464

Y262A,Y632A,Y1464A, coordinates relative to Ncoa6-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Oh et al., 2014)
FBrf0233980-2.PD.WB
Reference
Oh et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

GST,V5 tag

yki 
yki 
Ncoa6 

Ncoa6

prey
Ncoa6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
C-terminal region
sufficient binding region
aa 241-418

coordinates relative to yki-PH

yki 
WW domain
mutation disrupting interaction
aa 292,295,361,364

W292A,P295A,W361A,P364A, coordinates relative to yki-PH

Ncoa6 
interaction domain
mutation disrupting interaction
aa 262,632,1464

Y262A,Y632A,Y1464A, coordinates relative to Ncoa6-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from nuclear S2 cell extract.

(Oh et al., 2014)
FBrf0247927-12.coIP.WB
Reference
Srivastava et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Firefly Luciferase tag

Ncoa6 

NcoA6

prey

Renilla Luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Yki2/yki-PF isoform including exon 3 interacted more strongly with Ncoa6 than an isoform lacking exon 3.

(Srivastava et al., 2021)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Srivastava et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)