"ΔPRM" construct, coordinates relative to mbc-PA
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
This construct lacks the C terminal PXXP motifs and also has P to A mutations as aa 110 and aa 113
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.
MBC SH3 domain was also cloned and used as a negative control.
This construct lacks the C terminal PXXP motifs and also has P to A mutations at aa 110 and aa 113
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Authors conclude that either isoform of Crk can interact strongly with MBC and neither is capable of binding to MBC in the absence of this region.
