FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Tbp, Taf2
FlyBase ID
FBig0000102678
Interaction Network
Interactions Browser links
Tbp network
Taf2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0065619-2.coIP.MW
Reference
Yokomori et al., 1993
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

TBP

bait
Taf2 

dTAFII150

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Yokomori et al., 1993)
FBrf0074707-13.coIP.A
Reference
Verrijzer et al., 1994
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

dTBP

bait

HA tag

Taf2 

dTAF[[II]]150

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 cell system; prey produced and labeled by in vitro translation.

(Verrijzer et al., 1994)

Taf2 binds Tbp and Taf1 but not Taf4 in vitro.

(Verrijzer et al., 1994)
FBrf0074707-14.coIP.WB
Reference
Verrijzer et al., 1994
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

dTBP

bait

HA tag

Taf2 

dTAF[[II]]150

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Verrijzer et al., 1994)
FBrf0074707-2.coIP.MW
Reference
Verrijzer et al., 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

TBP

bait
Taf2 

dTAF[[II]]150

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Verrijzer et al., 1994)
FBrf0078200-2.coIP.MW
Reference
Chen et al., 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

dTFIID

bait
Taf2 

dTAF[[II]]150

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Chen et al., 1994)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)