Prey was cloned reagent (previously isolated).
Co-transfection of S2 cells with tagged bait and tagged prey constructs.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Embryos were paraformaldehyde crosslinked before affinity purification.
Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Embryos were paraformaldehyde crosslinked before affinity purification; weak interaction.
Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
