FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Taf2, Taf6
FlyBase ID
FBig0000102529
Interaction Network
Interactions Browser links
Taf2 network
Taf6 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0074707-11.coIP.WB
Reference
Verrijzer et al., 1994
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Taf2 

dTAF[[II]]150

bait
Taf6 

dTAF[[II]]60

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Verrijzer et al., 1994)
FBrf0209543-92.coIP.MS
Reference
Weake et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Taf6 

TAF6

bait

FLAG tag, HA tag

Taf2 

TAF2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Weake et al., 2009)

The Drosophila TFIID complex is composed of the following subunits: e(y)1, (Taf9), Taf10b, Taf12, Taf1, Taf2, bip2, Taf4, Taf5, Taf6, Taf7, Taf8, Taf10, Taf11, Taf13, Tbp, Trf and Trf2@.

(Weake et al., 2009)

In Drosophila, Taf6 is restricted to the TFIID complex and is not found in the Drosophila SAGA complex.

(Weake et al., 2009)

Tandem affinity purification of bait and identification of prey by MudPIT.

(Weake et al., 2009)

Positive control.

(Weake et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)