11 out of 12 positive clones pulled out of the SuUR two hybrid screen corresponded to Su(var)205.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast LexA-BD/B42-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
SuUR interacted with Su(var)205 (HP1), but not Su(var)3-7, Su(var)3-9 or Pc in this assay.
Two-hybrid system: yeast LexA-BD/B42-AD
Two-hybrid system: yeast LexA-BD/B42-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic nuclear extract.
The central region of SuUR pulls down Su(var)205 (HP1) even more efficiently than does full length SuUR.
Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Crude nuclear extracts were cross-linked using formaldehyde, sonicated, and subjected to ProteinA affinity purification with rabbit IgG agarose beads eluted under denaturing conditions, and subsequently using streptavidin agarose beads for Bio affinity purification. Purified peptides were eluted by on-bead trypsin digestion and mass spectrometric analysis.
Enrichment of prey protein was compared to input, mock and unrelated BioTAP affinity purifications using a statistical method (Bamse) developed by the authors to control for multiple sources of bias.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.