Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
The Drosophila SAGA complex is composed of the following subunits: Gcn5, Ada2b, dik, Sgf29, CG6506, Ada1-2, Spt3, Spt20, Nipped-A, e(y)1 (Taf9), Taf10b, Taf12, wda, Saf6, not, Sgf11, e(y)2 and Atxn7.
Tandem affinity purification of bait and identification of prey by MudPIT.