FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Polr2A, Polr2B
FlyBase ID
FBig0000102288
Interaction Network
Interactions Browser links
Polr2A network
Polr2B network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0224244-24.coIP.WB
Reference
Benbahouche et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Polr2A 

Rpb1

bait
Polr2B 

Rpb2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Benbahouche et al., 2014)

Positive control.

(Benbahouche et al., 2014)
FBrf0257712-1.coIP.MW
Reference
Dollinger et al., 2023
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Polr2A 

Rbp1

bait

FLAG tag

Polr2B 

Rbp2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extracts of wild-type fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Dollinger et al., 2023)

FLAG tagged Polr2A was used to purify the RNA polymerase II complex. Captured as pairwise interactions.

(Dollinger et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)