FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pldn, Dysb
FlyBase ID
FBig0000102225
Interaction Network
Interactions Browser links
Pldn network
Dysb network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0209903-1.Y2H
Reference
Cheli et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pldn 

Pallidin

bait

fused to GAL4 DNA-binding domain

Dysb 

Dysbindin

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Cheli et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Cheli et al., 2010)
FBrf0227264-3.coIP.MS
Reference
Mullin et al., 2015
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dysb 

Dsybindin

bait

Venus tag

Pldn 

Pallidin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Dysb, Snapin, Muted, Pallidin, Blos1, Blos2, Blos3 and Blos4 constitute the BLOC-1 octameric complex.

(Mullin et al., 2015)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Mullin et al., 2015)
FBrf0227264-8.coIP.WB
Reference
Mullin et al., 2015
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dysb 

Dsybindin

bait

Venus tag

Pldn 

Pallidin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Mullin et al., 2015)
FBrf0227264-9.coIP.WB
Reference
Mullin et al., 2015
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pldn 

Pallidin

bait
Dysb 

Dsybindin

prey

Venus tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Mullin et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)