Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Sequential GST pull down and anti-FLAG coimmunoprecipitation of coexpressed proteins.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Sequential GST pull down and anti-FLAG coimmunoprecipitation of coexpressed proteins.

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The Drosophila SAGA complex is composed of the following subunits: Gcn5, Ada2b, dik, Sgf29, CG6506, Ada1-2, Spt3, Spt20, Nipped-A, e(y)1 (Taf9), Taf10b, Taf12, wda, Saf6, not, Sgf11, e(y)2 and Atxn7.

In Drosophila, Saf6 interacts with Taf9, replacing Taf6, and functioning analogously to TAF6/TAF6L in the yeast and human SAGA complexes.

Tandem affinity purification of bait and identification of prey by MudPIT.