FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sxl, nanos
FlyBase ID
FBig0000101968
Interaction Network
Interactions Browser links
Sxl network
nanos network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0218549-1.coIP.PCR
Reference
Chau et al., 2012
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sxl 

Sxl

bait
nanos 

nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries; bait produced from endogenous gene; prey produced from endogenous gene.

(Chau et al., 2012)
FBrf0218549-2.coIP.PCR
Reference
Chau et al., 2012
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sxl 

Sxl

bait
nanos 

nos

prey

Myc tag

nanos 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
S5,S1,S3 binding sites
mutation decreasing interaction
unspecified

S5 site AUUUUUUUU to AUCUCUCUC, S1 site UUUUUUUUU TO UCUCUCUCU, S3 site AUUUUUUUUAUUUUUUU TO AUCUCUCUCAUCUCUCU

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries; bait produced from endogenous gene; prey produced from transgenic construct.

(Chau et al., 2012)
FBrf0221101-7.coIP.PCR
Reference
Li et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sxl 

Sxl

bait
nanos 

nanos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2013)
FBrf0239135-2.CLIP.PCR
Reference
Morita et al., 2018
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sxl 

Sxl

bait

GFP tag

nanos 

nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Morita et al., 2018)

Source was adult bam mutant ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Morita et al., 2018)
FBrf0247403-14.coIP.PCR
Reference
Malik et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sxl 

Sxl

bait

HA tag

nanos 

Nos

prey
nanos 
nanos 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3-prime UTR distal
sufficient binding region
nt 781-851
nanos 
3-prime UTR Sxl binding site
mutation disrupting interaction
nt 787-800

UUUUUUUAUUUUUU to UUUCUCUAUCUCUU

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Malik et al., 2020)
FBrf0247403-7.coIP.PCR
Reference
Malik et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sxl 

Sxl

bait

HA tag

nanos 

Nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Malik et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)