FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nanos, mei-P26
FlyBase ID
FBig0000101871
Interaction Network
Interactions Browser links
nanos network
mei-P26 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0218009-6.coIP.WB
Reference
Li et al., 2012
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

nos

bait

Myc tag

mei-P26 

mei-P26

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2012)

Interaction detected in wild-type and bam mutant ovaries.

(Li et al., 2012)
FBrf0221101-8.coIP.PCR
Reference
Li et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mei-P26 

Mei-P26

bait
nanos 

nanos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2013)
FBrf0247403-12.coIP.PCR
Reference
Malik et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mei-P26 

Mei-P26

bait

Myc tag

nanos 

Nos

prey
nanos 
nanos 
nanos 
nanos 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3-prime UTR proximal
sufficient binding region
nt 21-100
nanos 
3-prime UTR miRNA binding sites
mutation disrupting interaction
nt 34-40,76-81

GGCAGCU to GGUAGUU and CAUUCC to CAUUUU

nanos 
3-prime UTR distal
sufficient binding region
nt 781-851
nanos 
3-prime UTR Sxl binding site
mutation disrupting interaction
nt 787-800

UUUUUUUAUUUUUU to UUUCUCUAUCUCUU

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Malik et al., 2020)
FBrf0247403-5.coIP.PCR
Reference
Malik et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mei-P26 

Mei-P26

bait

Myc tag

nanos 

Nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Malik et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)