Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

The da-l(1)sc interaction is stronger than da-ac, which is stronger than da-sc.

Two-hybrid system: yeast, using a lacZ reporter under the control of hb regulatory sequence.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

A [32]P labeled DNA probe consisting of an E box from a ase regulatory fragment was was mixed with da and/or l(1)sc. Cooperative binding to the DNA was observed by EMSA.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Two-hybrid system: yeast, using a lacZ reporter under the control of hb regulatory sequence.

The hb reporter construct responds more strongly to the da-sc combination than to the da-l(1)sc combination, or to da alone. sc or l(1)sc alone do not have activity.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

A [32]P labeled DNA probe was mixed with da and/or l(1)sc. Cooperative binding to the DNA was observed by EMSA.

da-l(1)sc form a protein:DNA complex that is distinct from complexes observed in the presence of da l(1)sc or alone.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from genomic expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

da-l(1)sc form a protein:DNA complex that is distinct from complexes observed in the presence of da l(1)sc or alone.

A [32]P labeled DNA probe was mixed with da and/or l(1)sc. Cooperative binding to the DNA was observed by EMSA.