Twi
Da
Interaction in vitro; proteins produced by in vitro translation.
Electrophoretic mobility shift assays (EMSA) revealed that twi/da form heterodimers on either a CATATG or CACCTG sequences derived from the rho gene.
Twist
GST tag
Daughterless
[35]S label
GST pull down.
Positive control.
Bait expressed and purified from bacteria.
Prey derived from in vitro translation.
