Source was embryos of wild-type fly line; proteins produced from endogenous genes.
The structure of the Drosophila 80S ribosome with accessory proteins solved by cryo-EM. Complex captured as 3828 pairwise interactions which do not necessarily reflect direct contact.
Source was live S2 cells; bait produced from transfected construct; prey produced from transfected construct.
To visualize the association of the 40S and 60S ribosomal subunits, a protein in each subunit was tagged with a complementary fragment of the fluorescent Venus protein. Proximity of the two proteins from different ribosomal subunits resulted in reconstitution of the fluorescent protein.
Developed as a technique to detect assembled 80S ribosomes and potential active translation in the nervous system.
Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.
