FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dco, Bdbt
FlyBase ID
FBig0000100801
Interaction Network
Interactions Browser links
dco network
Bdbt network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0223409-1.coIP.MS
Reference
Fan et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

Myc tag

dco 
Bdbt 

CG17282

prey

5 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dco 
C-terminal region
necessary binding region
aa 296-440
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Fan et al., 2013)

Bdbt was identified with full-length or kinase-dead dco, but not with C-terminally truncated forms of dco.

(Fan et al., 2013)
FBrf0223409-2.PD.TV
Reference
Fan et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

GST tag

Bdbt 

CG17282

prey

fluorescent lysine label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bdbt interacts with dco but not per in vitro.

(Fan et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Fan et al., 2013)
FBrf0223409-3.coIP.WB
Reference
Fan et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

Myc tag

Bdbt 

CG17282

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Fan et al., 2013)
FBrf0223409-4.coIP.WB
Reference
Fan et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

dbt

bait

Myc tag

Bdbt 

CG17282

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Fan et al., 2013)
FBrf0223409-5.PD.WB
Reference
Fan et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

GST tag

Bdbt 

CG17282

prey
Bdbt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Bdbt 
N-terminal region
sufficient binding region
aa 1-120
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fan et al., 2013)
FBrf0228956-1.coIP.WB
Reference
Venkatesan et al., 2015
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

Myc tag

Bdbt 

BDBT

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Venkatesan et al., 2015)
FBrf0241476-1.coIP.WB
Reference
Venkatesan et al., 2019
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

Myc tag

dco 
dco 
Bdbt 

BDBT

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dco 
NLS domain
mutation disrupting interaction
aa 221,222

K221N,R222N

dco 
interaction domain
mutation disrupting interaction
aa 192

R192N

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Venkatesan et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)